ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of Gansui Banxia Tang plus-minus Gansui and Gancao anti-drug combination on hepatic and renal functions in malignant ascites rats to explore whether the efficacy or toxicity associated with the anti-drug combination.</p><p><b>METHOD</b>The male wistar rats were randomly divided into a blank group, model group, furosemide group, Gansui Banxia Tang group, Gansui Banxia Tang removed Zhigancao group, Gansui Banxia Tang removed Cugansui group, Gansui Banxia Tang removed Zhigancao and Cugansui group. In addition to normal feeding, every morning except for the blank group and model group, the rest of the group was given drugs, the control group and the model group was given distilled water, the volume is 10 mL x kg(-1). Administered five days, all rats were fasted but except water for 24 hours to collect urine. Administered nine days all rats were fasted but except water for 12 hours, we need to weigh weight of rats. When we remove the ascites, we also need to weigh weight of rats. We use the weight before removing ascites minus weight after removing ascites to indirectly measure the amount of ascites. When we remove the ascites, we need to abdominal aortic blood, centrifuge testing renin, angiotensin II, aldosterone, antidiuretic hormone and other indicators.</p><p><b>RESULT</b>The effect of Gansui Banixa Tang on increasing the net weight, lowering abdominal circumference and body weight ratio, lowering renin, angiotensin, aldosterone, antidiuretic hormone is better than the other treatment group.</p><p><b>CONCLUSION</b>In diuresis party, the group of Gansui Banxia Tang is better than the group of Gansui Banxia Tang remove Zhigancao or Cugansui or Zhigancao and Cugansui, renin-angiotensin-aldosterone system may play a diuretic effect of its one way.</p>
Subject(s)
Animals , Male , Rats , Aldosterone , Metabolism , Angiotensin II , Metabolism , Ascites , Drug Therapy , Metabolism , Body Weight , Diuretics , Pharmacology , Therapeutic Uses , Dose-Response Relationship, Drug , Drug Interactions , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Rats, Wistar , Renin-Angiotensin SystemABSTRACT
<p><b>OBJECTIVE</b>To investigate the association between 1858C/T single nucleotide polymorphism on exon 14 of the protein tyrosine phosphatase nonreceptor-22 (PTPN22) gene and systemic lupus erythematosus in Chinese Han patients.</p><p><b>METHODS</b>Forty Chinese Han patients with SLE and 20 normal control subjects were enrolled in the study, and PTPN22 gene 1858 C/T polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).</p><p><b>RESULTS</b>No significant difference was found in the CC, CT, and TT genotype frequencies or in the C and T allele frequencies between the SLE patients and the normal controls.</p><p><b>CONCLUSION</b>1858C/T on PTPN22 gene might not be associated with the susceptibility of SLE in Chinese Han population.</p>
Subject(s)
Adolescent , Adult , Child , Female , Humans , Male , Middle Aged , Young Adult , Asian People , Genetics , Case-Control Studies , Exons , Gene Frequency , Genetic Predisposition to Disease , Genotype , Lupus Erythematosus, Systemic , Genetics , Polymorphism, Single Nucleotide , Protein Tyrosine Phosphatase, Non-Receptor Type 22 , GeneticsABSTRACT
This article reviews rectification and protection of Chinese patent medicines, establishment and improvement of the new drug approval, and historical evolution of the pharmacopoeia of the People's Republic of China. The article analyzes the present situation and the challenges of quality standardization of Chinese patent medicines in the new era. The article also points out strategies and guidelines to be taken to promote the globalization of Chinese patent medicines and compliance with the international standards.
Subject(s)
China , Drug Approval , Drug Contamination , Drugs, Chinese Herbal , Reference Standards , Nonprescription Drugs , Reference Standards , Pharmacopoeias as Topic , Reference Standards , Quality ControlABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Qilan Tangzhining capsule on glucose and lipid metabolism in diabetes mellitus and hyperlipemia.</p><p><b>METHOD</b>Rats were fed with high sugar and fat food for 2 months, then 2% streptozocin (STZ, 30 mg kg(-1)) was injected intraperitoneally to induce hyperglycemia and hyperlipemia. After the model rats were administrated drugs, the blood glucose, the serum lipids, and the histopathology of the liver and pancreas were observed.</p><p><b>RESULT</b>Qilan Tangzhining capsule could decrease the FBG and the serum TC, TG, LDL-C, increase HDL-C, and improve the histopathologic injury of the liver and pancreas.</p><p><b>CONCLUSION</b>Qilan Tangzhining capsule can not only adjust the blood glucose and lipid, but also improve the histopathology injury of the liver and pancreas.</p>
Subject(s)
Animals , Male , Rats , Astragalus propinquus , Chemistry , Blood Glucose , Metabolism , Capsules , Diabetes Mellitus, Experimental , Blood , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Gynostemma , Chemistry , Hyperlipidemias , Blood , Lipids , Blood , Plants, Medicinal , Chemistry , Random Allocation , Rats, WistarABSTRACT
<p><b>OBJECTIVE</b>To investigate the behavioral changes and the levels of monoamine neurotransmitters in the anterior cortex in the olfactory bulb damage rats after being treated with Guanyu capsules (GYC).</p><p><b>METHOD</b>Open-field test and step-down passive avoidance test were used to observe the behavior in model rats. HPLC-ECD was used to analyze the influences of GYC on the levels of monoamine neurotransmitters.</p><p><b>RESULT</b>In the model rats, there was a characteristic hyperactivity in the Open-field and learning deficits in step-down passive avoidance (P < 0.01). The contents of 5-HT reduced, and the rate of DOPAC/DA increased significantly (P < 0.01). GYC 1.2, 0.6 g x kg(-1) could correct behavioral changes increase the contents of 5-HT, and decrease DOPAC/DA level (P < 0.01).</p><p><b>CONCLUSION</b>GYC can correct behavioral changes in rats model of olfactory bulb damage, and regulating 5-HT and DA metabolism in cortex is one of the antidepressive mechanisms of GYC.</p>
Subject(s)
Animals , Male , Rats , 3,4-Dihydroxyphenylacetic Acid , Metabolism , Behavior, Animal , Biogenic Monoamines , Metabolism , Cerebral Cortex , Metabolism , Curcuma , Chemistry , Depression , Metabolism , Dopamine , Metabolism , Drugs, Chinese Herbal , Pharmacology , Dryopteris , Chemistry , Olfactory Bulb , Pathology , Plants, Medicinal , Chemistry , Random Allocation , Rats, Sprague-Dawley , Serotonin , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To establish the determination method for complanatoside A in seeds of Astragalus complanatus.</p><p><b>METHOD</b>An HPLC method has been developed to separate complanatoside A on ZORBAX EXTEND-C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-water-phosphoric acid (20:80:0.2) as mobile phase and UV detection at 267 nm.</p><p><b>RESULT</b>The good linearity of complanatoside A ranged 0.086-0.430 microg, r = 0.9999. An average recovery of 99.8% (n = 5) was obtained with a RSD of 1.0%.</p><p><b>CONCLUSION</b>The established method is proved to be stability, fast, accurate and can be used for quantification of Complanatoside A in Semen Astragali Complanati.</p>